input: Isabella received the title of Catholic Monarch by Pope Alexander VI, a pope of whose behavior and involvement in matters Isabella did not approve. Along with the physical unification of Spain, Isabella and Ferdinand embarked on a process of spiritual unification, trying to bring the country under one faith (Roman Catholicism). As part of this process, the Inquisition became institutionalised. After a Muslim uprising in 1499, and further troubles thereafter, the Treaty of Granada was broken in 1502, and Muslims were ordered to either become Christians or to leave. Isabella's confessor, Cisneros, was named Archbishop of Toledo. He was instrumental in a program of rehabilitation of the religious institutions of Spain, laying the groundwork for the later Counter-Reformation. As Chancellor, he exerted more and more power.  Isabella and her husband had created an empire and in later years were consumed with administration and politics; they were concerned with the succession and worked to link the Spanish crown to the other rulers in Europe. By early 1497, all the pieces seemed to be in place: The son and heir John, Prince of Asturias, married a Habsburg princess, Margaret of Austria, establishing the connection to the Habsburgs. The eldest daughter, Isabella of Aragon, married King Manuel I of Portugal, and the younger daughter, Joanna of Castile, was married to a Habsburg prince, Philip I of Habsburg.  However, Isabella's plans for her eldest two children did not work out. Her only son, John of Asturias, died shortly after his marriage. Her daughter Isabella of Aragon, whose son Miguel da Paz died at the age of two, died in childbirth. Queen Isabella I's crowns passed to her third child Joanna and her son-in-law, Philip I.  Isabella did, however, make successful dynastic matches for her three youngest daughters. The death of Isabella of Aragon created a necessity for Manuel I of Portugal to remarry, and Isabella's third daughter, Maria of Aragon, became his next bride. Isabella's youngest daughter, Catherine of Aragon, married England's Arthur, Prince of Wales, but his early death resulted in her being married to his younger brother, King Henry VIII of England.  Isabella officially withdrew from governmental affairs on September 14, 1504 and she died that same year on 26 November at the Medina del Campo Royal Palace. It is said that she had truly already been in decline since the death of her son Prince John of Asturias in 1497. She is entombed in Granada in the Capilla Real, which was built by her grandson, Charles V, Holy Roman Emperor (Carlos I of Spain), alongside her husband Ferdinand, her daughter Joanna and Joanna's husband Philip I; and Isabella's 2-year-old grandson, Miguel da Paz (the son of Isabella's daughter, also named Isabella, and King Manuel I of Portugal). The museum next to the Capilla Real holds her crown and scepter.

Answer this question "Do they have any kids?"
output: Isabella's plans for her eldest two children did not work out.

input: The study of Molaison revolutionized the understanding of the organization of human memory. It has provided broad evidence for the rejection of old theories and the formation of new theories on human memory, in particular about its processes and the underlying neural structures (cf. Kolb & Whishaw, 1996). In the following, some of the major insights are outlined.  Molaison's brain was the subject of an anatomical study funded by the Dana Foundation and the National Science Foundation. The aim of the project, headed by Jacopo Annese, of The Brain Observatory at UC San Diego, was to provide a complete microscopic survey of the entire brain to reveal the neurological basis of Molaison's historical memory impairment at cellular resolution. On December 4, 2009, Annese's group acquired 2401 brain slices, with only two damaged slices and 16 potentially problematic slices. The digital 3D reconstruction of his brain was finished at the beginning of 2014.  The results of the study were published in Nature Communications for January 2014. The researchers found, to their surprise, that half of H.M.'s hippocampus had survived the 1953 surgery, which has deep implications on past and future interpretations of H.M.'s neurobehavioral profile and of the previous literature describing H.M. as a 'pure' hippocampus lesion patient. Additionally, a previously unexpected discrete lesion was discovered in the prefrontal cortex. These findings suggest revisiting raw data from behavioral testing. A three-dimensional virtual model of the brain allowed the dynamics of the surgery to be reconstructed; it was found that the brain damage above the left orbit could have been created by Dr. Scoville when he lifted the frontal lobe to reach into the medial temporal lobes.  The article also describes the general neuropathological state of the brain via multiple imaging modalities. As H.M. was 82 when he died, his brain had aged considerably. Several pathological features were discovered, some severe, which had contributed to his cognitive decline.  The digital atlas of HM's brain was made publicly available on the Internet free of charge; its "permanence on the web relies on contributions from users".

Answer this question "What were the final conclusions about his brain?"
output:
The researchers found, to their surprise, that half of H.M.'s hippocampus had survived the 1953 surgery,