On December 8, 2004, during the Devastation Across The Nation tour, Abbott was shot on-stage while performing with Damageplan at the Alrosa Villa in Columbus, Ohio. A crowd of approximately 250 had watched four support acts (two local bands entitled Volume Dealer and 12 Gauge, and the tour support Shadows Fall and The Haunted). Moments into Damageplan's set, 25-year-old former Marine Nathan Gale shot Abbott five times in the head with a 9 mm Beretta 92F pistol. Some in attendance initially believed the shooting was part of the act, but as Gale continued shooting, the audience quickly came to the realization that the event was not staged. Firing a total of 15 shots, Gale killed three other people and wounded seven more.  Jeff "Mayhem" Thompson, the band's head of security, was killed tackling Gale, as was Alrosa Villa employee Erin Halk. Audience member Nathan Bray was killed while trying to perform CPR on Abbott and Thompson. It was rumored that one crowd member leapt in front of the gunman, saving the lives of several band members. Damageplan's drum technician, John "Kat" Brooks, was shot three times as he attempted to disarm Gale, but was overpowered and taken hostage in a headlock hold. Tour manager Chris Paluska was also injured.  Responding within three minutes to a dispatch call made at 10:15pm, seven police officers entered through the front entrance and moved toward the stage. Officer James Niggemeyer came in through the back door, behind the stage. Gale only saw the officers in front of the stage; he did not see Niggemeyer, who was armed with a 12 gauge Remington 870 shotgun. Niggemeyer approached Gale from the opposite side of the stage past a group of security guards, and saw Gale lift his gun to Brooks' head, and fired a single shot as Gale noticed him. Gale was struck in the face with eight of the nine buckshot pellets and was killed instantly. Gale was found to have had 35 rounds of ammunition remaining.  Two fans, including Mindy Reece, a certified nurse, administered CPR on Abbott until paramedics arrived, but were unable to revive him and he was pronounced dead at the scene. Early speculation about motive suggested that Gale, who was a Pantera fan, might have turned to violence in response to the breakup of the band, or the public dispute between Abbott and Pantera singer Phil Anselmo, but these were later ruled out by investigators. In VH1's documentary, Behind the Music, Damageplan's sound engineer Aaron Barnes stated that the whole time, after shooting Dimebag, Gale was looking for Vinnie, possibly planning to murder him too. Another conjecture was that Gale believed Abbott had stolen a song that he had written. About six months prior to the shooting, Gale got into an altercation at a Damageplan concert in Cincinnati where he damaged $5,000 worth of equipment while being removed from the stage by security.

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