Question:
Henrietta Lacks (born Loretta Pleasant; August 1, 1920 - October 4, 1951) was an African-American woman whose cancer cells are the source of the HeLa cell line, the first immortalized cell line and one of the most important cell lines in medical research. An immortalized cell line will reproduce indefinitely under specific conditions, and the HeLa cell line continues to be a source of invaluable medical data to the present day. Lacks was the unwitting source of these cells from a tumor biopsied during treatment for cervical cancer at Johns Hopkins Hospital in Baltimore, Maryland, U.S., in 1951. These cells were then cultured by George Otto Gey who created the cell line known as HeLa, which is still used for medical research.
George Otto Gey, the first researcher to study Lacks's cancerous cells, observed that her cells were unique in that they reproduced at a very high rate and could be kept alive long enough to allow more in-depth examination. Until then, cells cultured for laboratory studies survived for only a few days at most, which wasn't long enough to perform a variety of different tests on the same sample. Lacks's cells were the first to be observed that could be divided multiple times without dying, which is why they became known as "immortal." After Lacks' death, Gey had Mary Kubicek, his lab assistant, took further HeLa samples while Henrietta's body was at Johns Hopkins' autopsy facility. The roller-tube technique was the method used to culture the cells obtained from the samples that Kubicek collected. Gey was able to start a cell line from Lacks's sample by isolating one specific cell and repeatedly dividing it, meaning that the same cell could then be used for conducting many experiments. They became known as HeLa cells, because Gey's standard method for labeling samples was to use the first two letters of the patient's first and last names.  The ability to rapidly reproduce HeLa cells in a laboratory setting has led to many important breakthroughs in biomedical research. For example, by 1954, Jonas Salk was using HeLa cells in his research to develop the polio vaccine. To test his new vaccine, the cells were mass-produced in the first-ever cell production factory. Additionally, Chester M. Southam, a leading virologist, injected HeLa cells into cancer patients, prison inmates, and healthy individuals in order to observe whether cancer could be transmitted as well as to examine if one could become immune to cancer by developing an acquired immune response.  HeLa cells were in high demand and put into mass production. They were mailed to scientists around the globe for "research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits". HeLa cells were the first human cells successfully cloned in 1955, and have since been used to test human sensitivity to tape, glue, cosmetics, and many other products. Since the 1950s, scientists have grown 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.  In the early 1970s, a large portion of other cell cultures became contaminated by HeLa cells. As a result, members of Henrietta Lacks's family received solicitations for blood samples from researchers hoping to learn about the family's genetics in order to differentiate between HeLa cells and other cell lines.  Alarmed and confused, several family members began questioning why they were receiving so many telephone calls requesting blood samples. In 1975, the family also learned through a chance dinner-party conversation that material originating in Henrietta Lacks was continuing to be used for medical research. The family had never discussed Henrietta's illness and death among themselves in the intervening years but with the increased curiosity about their mother and her genetics, they now began to ask questions.
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How did Gey make his discovery?

Answer:
After Lacks' death, Gey had Mary Kubicek, his lab assistant, took further HeLa samples while Henrietta's body was at Johns Hopkins' autopsy facility.


Question:
The Joker is a fictional supervillain created by Bill Finger, Bob Kane, and Jerry Robinson who first appeared in the debut issue of the comic book Batman (April 25, 1940), published by DC Comics. Credit for the Joker's creation is disputed; Kane and Robinson claimed responsibility for the Joker's design, while acknowledging Finger's writing contribution. Although the Joker was planned to be killed off during his initial appearance, he was spared by editorial intervention, allowing the character to endure as the archenemy of the superhero Batman. In his comic book appearances, the Joker is portrayed as a criminal mastermind.
The Joker debuted in Batman #1 (Spring 1940) as the eponymous character's first villain, shortly after Batman's debut in Detective Comics #27 (May 1939). The Joker initially appeared as a remorseless serial killer, modeled after a joker playing card with a mirthless grin, who killed his victims with "Joker venom": a toxin which left their faces smiling grotesquely. The character was intended to be killed in his second appearance in Batman #1, after being stabbed in the heart. Finger wanted the Joker to die because of his concern that recurring villains would make Batman appear inept, but was overruled by then-editor Whitney Ellsworth; a hastily drawn panel, indicating that the Joker was still alive, was added to the comic. The Joker went on to appear in nine of Batman's first twelve issues.  The character's regular appearances quickly defined him as the archenemy of the dynamic duo, Batman and Robin; he killed dozens of people, and even derailed a train. By issue #13, Kane's work on the syndicated Batman newspaper strip left him little time for the comic book; artist Dick Sprang assumed his duties, and editor Jack Schiff collaborated with Finger on stories. Around the same time, DC Comics found it easier to market its stories to children without the more mature pulp elements that had originated many superhero comics. During this period, the first changes in the Joker began to appear, portraying him more as a prankster than threat; when he kidnaps Robin, Batman pays the ransom by check, meaning that the Joker cannot cash it without being arrested. Comic book writer Mark Waid suggests that the 1942 story "The Joker Walks the Last Mile" was the beginning point for the character's transformation into a more goofy incarnation, a period that Grant Morrison considered lasted the following thirty years.  The 1942 cover of Detective Comics #69, known as "Double Guns" (with the Joker emerging from a genie lamp, aiming two guns at Batman and Robin), is considered one of the greatest superhero comic covers of the Golden Age and is the only image of the character using traditional guns. Robinson said that other contemporary villains used guns, and the creative team wanted the Joker--as Batman's adversary--to be more resourceful.
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What was the Joker first introduced as?

Answer:
). The Joker initially appeared as a remorseless serial killer,