On June 30, 2009, McKinney was aboard the Greek-flagged Free Gaza Movement's ship Spirit of Humanity carrying 21 activists including Irish peace activist Mairead McGuire, medical supplies, a symbolic bag of cement, olive trees and toys, when it was seized by the Israeli Navy 18 mi (29 km) off the Gaza coast. It is unclear whether they were in international waters or in Gazan waters, which is subject to the Israeli blockade of Gaza. Although both the Cypriot and Israeli authorities were officially informed the destination was Gaza before the vessel's departure, according to the Cypriot government the ship "was given permission by the competent Authorities of the Republic of Cyprus to sail off the port of Larnaca in Cyprus on the basis of its declaration that its intended destination was the port of Port Said in Egypt."  McKinney was held at the Givon immigration detention center in Ramle, until she was released on July 5. McKinney initially refused to sign the deportation papers because they were written in Hebrew and that the papers would require them to admit that they were in violation of Israel's blockade, which they deny. According to The Atlanta Journal-Constitution, Israeli officials stated that the "Palestinian Authority and the rest of the international community had agreed to the off-shore blockade to prevent arms smuggling into Gaza." The Palestinian Chronicle reports that such an agreement to the off-shore blockade never happened. "No Palestinians have agreed nor did the international community agree to a blockade of Gaza by land or Sea." On June 17, 2009, a group of United Nations agencies and non-governmental organizations (NGOs) called for an end to Israel's blockade of the Gaza Strip.  On July 7, 2009, McKinney was deported to the United States. The Israeli government indicated it will deliver the supplies via land.

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