Born Marilyn Ann Briggs in Providence, Rhode Island, Chambers was raised in Westport, Connecticut, in a middle-class household. It is often reported that she was born in Westport; however, in a 2007 interview Chambers confirmed she was born in Providence but grew up in Westport. Her father was in advertising and her mother was a nurse. She was the youngest of three children, including a brother, Martin Briggs (keyboardist for 1960s Boston band The Remains), and a sister, Jann Smith.

Chambers dreamed of having a career in mainstream films and believed her celebrity as the star of Behind the Green Door and the Ivory Snow girl would be a stepping stone to other endeavors. "The paradox was that, as a result of Green Door, Hollywood blackballed me," she said later. "[Green Door] became a very high-grossing film...But, to a lot of people, it was still a dirty movie; for me to do anything else, as an actress, was totally out of the question. I became known as a porno star, and that type of labeling really hurt me. It hurt my chances of doing anything else".  Throughout the 1970s she was up for roles in several Hollywood films. Her biggest opportunity came in 1976 when it was announced in Variety that she was to star alongside Rip Torn in City Blues, a film about a young hooker defended by a seedy lawyer. The film was to be directed by Nicholas Ray. Ray had never seen Behind the Green Door or even screen-tested Chambers. Instead the two met and Ray was impressed. "I have a camera in my head," he said, adding that Chambers would "eventually be able to handle anything that the young Katie Hepburn or Bette Davis could." However, the project never came to fruition, in large part due to Ray's alcohol and drug abuse.  Chambers claimed that Jack Nicholson and Art Garfunkel brought her in to talk about a role in the 1978 film Goin' South, then asked her for cocaine and grilled her about whether her orgasms in Behind the Green Door were real; she was angered to the point where she stormed out of the interview. She was going to be cast in the film Hardcore, opposite George C. Scott, but the casting director took one look at her and said she was too wholesome to be cast as a porn queen. "The Hardcore people wanted a woman with orange hair who chews gum, swings a big purse, and wears stiletto heels. That's such a cliche," Chambers said years later. Season Hubley was cast instead.

When did she leave Hollywood?