Question:
The overseas expansion under the crown of Castile was initiated under the royal authority and first accomplished by the Spanish conquistadores. The Americas were incorporated into the Spanish Empire, with the exception of Brazil and Canada, and the crown created civil and religious structures to administer the region. The motivations for colonial expansion were trade and the spread of the Catholic faith through indigenous conversions. Beginning with the 1492 arrival of Christopher Columbus in the Caribbean and continuing control of vast territory for over three centuries, the Spanish Empire would expand across the Caribbean Islands, half of South America, most of Central America and much of North America (including present day Mexico, Florida and the Southwestern and Pacific Coastal regions of the United States).
During the Napoleonic Peninsular War in Europe between France and Spain, assemblies called juntas were established to rule in the name of Ferdinand VII of Spain. The Libertadores (Spanish and Portuguese for "Liberators") were the principal leaders of the Spanish American wars of independence. They were predominantly criollos (Americas-born people of European ancestry, mostly Spanish or Portuguese), bourgeois and influenced by liberalism and in some cases with military training in the mother country.  In 1809 the first declarations of independence from Spanish rule occurred in the Viceroyalty of New Granada. The first two were in present-day Bolivia at Sucre (May 25), and La Paz (July 16); and the third in present-day Ecuador at Quito (August 10). In 1810 Mexico declared independence, with the Mexican War of Independence following for over a decade. In 1821 Treaty of Cordoba established Mexican independence from Spain and concluded the War. The Plan of Iguala was part of the peace treaty to establish a constitutional foundation for an independent Mexico.  These began a movement for colonial independence that spread to Spain's other colonies in the Americas. The ideas from the French and the American Revolution influenced the efforts. All of the colonies, except Cuba and Puerto Rico, attained independence by the 1820s. The British Empire offered support, wanting to end the Spanish monopoly on trade with its colonies in the Americas.  In 1898, the United States achieved victory in the Spanish-American War with Spain, ending the Spanish colonial era. Spanish possession and rule of its remaining colonies in the Americas ended in that year with its sovereignty transferred to the United States. The United States took occupation of Cuba, the Philippines, and Puerto Rico. Puerto Rico continues to be a possession of the United States, now officially continues as a self-governing unincorporated territory.
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How did America become colonized?

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Answer the question at the end by quoting:

Jonas Edward Salk (; October 28, 1914 - June 23, 1995) was an American medical researcher and virologist. He discovered and developed one of the first successful polio vaccines. Born in New York City, he attended New York University School of Medicine, later choosing to do medical research instead of becoming a practicing physician. In 1939, after earning his medical degree, Salk began an internship as a physician scientist at Mount Sinai Hospital.
In 1947, Salk became ambitious for his own lab and was granted one at the University of Pittsburgh, but the lab was smaller than he had hoped and he found the rules imposed by the university restrictive. In 1948, Harry Weaver, the director of research at the National Foundation for Infantile Paralysis, contacted Salk. He asked Salk to find out if there were more types of polio than the three then known, offering additional space, equipment and researchers. For the first year he gathered supplies and researchers including Julius Youngner, Byron Bennett, L. James Lewis, and secretary Lorraine Friedman joined Salk's team, as well. As time went on, Salk began securing grants from the Mellon family and was able to build a working virology laboratory. He later joined the National Foundation for Infantile Paralysis's polio project established by President Franklin D. Roosevelt.  Extensive publicity and fear of polio led to much increased funding, $67 million by 1955, but research continued on dangerous live vaccines. Salk decided to use the safer 'killed' virus, instead of weakened forms of strains of polio viruses like the ones used contemporarily by Albert Sabin, who was developing an oral vaccine. After successful tests on laboratory animals, on July 2, 1952, assisted by the staff at the D.T. Watson Home for Crippled Children, Salk injected 43 children with his killed-virus vaccine. A few weeks later, Salk injected children at the Polk State School for the retarded and feeble-minded. In 1954 he tested the vaccine on about one million children, known as the polio pioneers. The vaccine was announced as safe on April 12, 1955.  The project became large, involving 100 million contributors to the March of Dimes, and 7 million volunteers. The foundation allowed itself to go into debt to finance the final research required to develop the Salk vaccine. Salk worked incessantly for two and a half years.  Salk's inactivated polio vaccine was the first vaccine for the disease; it came into use in 1955. It is on the World Health Organization's List of Essential Medicines, the most effective and safe medicines needed in a health system.

Was the vaccine used?
Salk injected children at the Polk State School for the retarded and feeble-minded.